Journal: Nature Communications

Article Title: In situ valence-transited arsenic nanosheets for multi-modal therapy of colorectal cancer

doi: 10.1038/s41467-025-57376-7

Figure Lengend Snippet: a The percentages of DC maturation in tumors following different treatments (gated on CD11c + DC cells). b The percentages of tumoral CD4 + and CD8 + T cells following various treatments (gated on CD3 + T cells). The cytokines secretion of TNF-α ( c ) IFN-γ ( d ) and IL-1β ( e ) in serum following various treatments. The percentages ( f ) and statistical analysis ( g ) of M1 and M2 TAMs after different treatments (gated on F4/80 macrophages). h Immunofluorescent staining (M1-CD86) of tumor sections after different treatment. i The cytokine secretion of IL-6 and IL-10 in serum following different treatments. The gating strategy is presented in Fig. . For all figures here, data are presented as mean values +/− SD, n = 3 mice. One-way ANOVA with Tukey’s Multiple Comparison Test was used to compare the mean of each group with the mean of the Control group. P values were presented in absolute value in the figures.

Article Snippet: The tumor cell suspension was then stained with APC-F4/80 (Biolegend, Cat 123116), PE-CD86, FITC-CD206 (Biolegend, Cat 141703), APC-CD11 (Proteintech, Cat APC-65130), FITC-CD80 (Proteintech, Cat FITC-65076), FITC-CD3 (Proteintech, Cat FITC-65077), PE-CD8 (Proteintech, Cat PE-65068), and APC-CD4 (Proteintech, Cat APC-65141) antibodies for cell phenotype analysis by FCM.

Techniques: Staining, Comparison, Control

Journal: Current Microbiology

Article Title: Innate and Adaptive Immune Responses Induced by Aspergillus fumigatus Conidia and Hyphae

doi: 10.1007/s00284-022-03102-1

Figure Lengend Snippet: T cell expression of human PBMCs after stimulation by A. fumigatus conidia and hyphae. a CD4/CD8 ratio, b Th1, c Th2, d Th17. Each geometric symbol represents a sponsor ( n =4). Live Conidia LC, Live Hyphal fragments LH, Heat-inactivated Conidia HIC, Heat-inactivated Hyphal fragments HIH

Article Snippet: Next, cells were collected and washed with PBS, followed by extracellular staining with anti-human CD3 PE-Cy7, CD4 APC-Cy7 and CD8 PE-Cy5.5 for 15 min. After that, PBMCs were centrifuged and resuspended in Fixation/permeabilisation reagents (Biogems, USA) for 30 min to perform the subsequent intracellular cytokine staining with anti-human IL-4 R-PE, IL-17a APC and IFN-γ Alexa FlourTM 700 (all antibodies: BioLegend, USA).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Effects of different conditioning regimens on HLA-mismatched microtransplantation and changes in fine immune indices in acute myeloid leukaemia

doi: 10.1038/s41598-024-70332-7

Figure Lengend Snippet: Experimental reagents.

Article Snippet: APC-labelled Mouse Anti-Human CD4 Antibody , BD Bioscience, USA , 662965.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

doi: 10.1016/j.xpro.2024.103492

Figure Lengend Snippet: List of the markers used in the panel, and their description and purpose

Article Snippet: Anti-mouse CD4 APC-Cy5.5 (clone GK1.5); 1:2,000 , Abnova , Cat# MAB5925, RRID: AB_10558755.

Techniques: Marker, Migration, Glycoproteomics, Activation Assay, Functional Assay

Journal: STAR Protocols

Article Title: Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

doi: 10.1016/j.xpro.2024.103492

Figure Lengend Snippet: Intracellular staining mix

Article Snippet: Anti-mouse CD4 APC-Cy5.5 (clone GK1.5); 1:2,000 , Abnova , Cat# MAB5925, RRID: AB_10558755.

Techniques: Staining

Journal: STAR Protocols

Article Title: Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

doi: 10.1016/j.xpro.2024.103492

Figure Lengend Snippet:

Article Snippet: Anti-mouse CD4 APC-Cy5.5 (clone GK1.5); 1:2,000 , Abnova , Cat# MAB5925, RRID: AB_10558755.

Techniques: Purification, Recombinant, Centrifugation, Staining, Software